Pathogen-derived resistance to tomato spotted wilt virus in transgenic tomato and tobacco plants.

摘要

This study was undertaken to remedy significant yield losses in commercial tomato (Lycopersicon esculentum) and tobacco (Nicotiana tabacum) production caused by tomato spotted wilt virus (TSWV). One of the possible sources of resistance can be the incorporation into the host plant of a viral nucleoprotein (N) gene by Agrobacterium-mediated transformation. Twelve primary transformants of tomato and 141 of tobacco were analysed for the expression of the N gene and for resistance to the TSWV infection. The tests have demonstrated that transgenic plants were protected against virus infection irrespective of whether or not they contained detectable levels of the translational product.