In vitro Explant Growth and Shoot Regeneration from Petioles of Sugar Beet (Beta vulgaris L.) Lines at Different Ploidy Levels

摘要

This study was carried out to compare 'ELK 345' (diploid) and 'CBM 315' (tetraploid) sugar beet lines with respect to in vitro explant growth, shoot regeneration capacity of petiole explants, in vitro rooting and plantlet establishment. Petioles werecultured on MS (Murashige and Skoog) medium supplemented with 1 mg l~(-1) 6-benzylaminopurine (BAP) and 0.2 mg l~(-1) naph-thaleneacetic acid (NAA). Regenerated shoots were successfully rooted on MS medium containing 3 mg l~(-1) indole-3-butyric acid (B3A). It was observed that petiole explants of the tetraploid line 'CBM 315' gave rise to the highest scores with respect to explant fresh and dry weights, shoot regeneration percentage, shoot number per petiole, shoot length, total shoot number per Petridish, successful rooting and plantlet establishment. Petiole ex-plants of diploid line ('ELK 345') produced 12.61 shoots per ex-plants while petioles of tetraploid line ('CBM') produced 20.23 shoots per explant.