Inhomogeneity in Distribution and Conformation of Bovine Serum Albumin in Sol-Gel:A Closer Look with a Near-Infrared Multispectral Imaging Technique

摘要

Concentration distributions and conformations of bovine serum albumin (BSA) entrapped in sol-gels were successfully determined for the first time by use of the recently developed NIR multispectral imaging instrument.It was found that BSA molecules were inhomogeneously distributed within the sol-gel matrix,independent of its concentration.At relatively high concentration (366 mg/mL) the encapsulation process does not seem to produce any observable changes in the conformation of BSA.However,when the concentration of BSA was decreased to 220 mg/mL,pronounced changes in the spectra of the protein were observed as a function of (sol-gel reaction) time.The observed inhomogenity might be traced to both a non-uniform concentration distribution of BSA and changes in protein native conformation resulting from interactions between the charged protein and the silicate anions.Results obtained seem to suggest that the protein molecules might adapt themselves to accommodate evolving geometry and sites of the silica network.