Extension of the C18 stationary phase knowledge by using the carotenoid test

摘要

The carotenoid test for octadecylsiloxane-bonded stationary phases used in RPLC was developed some years ago. Additional experiments have now been performed with varied stationary phases. The effect of the bonding density and of the pore diameter on steric selectivity, polar surface activity and hydrophobicity was determined by using YMC series (J'Sphere and Pack ODSA). The test was also extended to estimate the phase evolution of several classical or hybrid silicas. The high loading phases were also studied, as well as the fused core silicas. The effect of the particle size reduction on the three previous phase properties was investigated in the goal to determine in which way this parameter could also modify the chemical properties of the phases.