Selenium speciation in tea by dispersive liquid-liquid microextraction coupled to high-performance liquid chromatography after derivatization with 2,3-diaminonaphthalene

摘要

Selenium is an important element for human health, and it is present in many natural drinks and foods. Present study described a new method using dispersive liquid-liquid microextraction prior to high-performance liquid chromatography with a UV variable wavelength detector for the determination of the total selenium, Se(IV), Se(VI), and total organoselenium in tea samples. In the procedure, 2,3-diaminonaphthalene was used as the chelating reagent, 400 mu L acetonitrile was used as the disperser solvent and 60 mu L chlorobenzene was used as the extraction solvent. The complex of Se(IV) and 2,3-diaminonaphthalene in the final extracted phase was analyzed by high-performance liquid chromatography. The factors influencing the derivatization and microextraction were investigated. Under the optimal conditions, the limit of detection was 0.11 mu g/L for Se(IV) and the linearity range was in the range of 0.5-40 mu g/L. This method was successfully applied to the determination of selenium in four tea samples with spiked recoveries ranging from 91.3 to 100%.