A NOVEL PEPTIDE DERIVED FROM HALIOTIS DISCUS HANNAI INHIBITS THE MIGRATION OF MKN-28 GASTRIC CANCER CELLS THROUGH DOWNREGULATION OF beta-CATENIN SIGNALING

摘要

Abalones are edible shellfish and a valuable food source in Asian countries. Although substances from abalone have bioactivities, such as anti-oxidation and anti-inflammation, anti-metastatic effects of abalone have not been fully revealed. Gastric cancer is a common malignant cancer, but prognosis is poor because of its high metastatic characteristics. In this study, a novel peptide (A2) from abalone (Haliotis discus hannai) was applied to investigate its antimetastatic effects in MKN-28 gastric cancer cells. Enhanced activities of glycogen-synthase kinase 3 beta(GSK-3 beta) by A2 treatment contributed to modulation of beta-catenin; hence, beta-catenin signaling was downregulated, and translocation of beta-catenin to the nucleus was repressed. Moreover, cellular protrusions including lamellipodia and filopodia were disrupted through downregulation of Rac1 and Cdc42 in response to A2 in MKN-28 cells. It is suggested that A2 inhibits cell migration through controlling levels of beta-catenin, GSK-3 beta, and also induced disorganization of lamellipodia and filopodia. Therefore, A2 possesses therapeutic properties to treat gastric cancer.