A Feed Serine Protease Improves Broiler Performance and Increases Protein and Energy Digestibility

摘要

Effects of a novel serine protease, RONOZYME (R) Pro Act (RPA) on protein solubilisation, hydrolysis and digestibility were tested in a gut simulation model. Furthermore, the effects of RPA (supplemented at 15,000 PROT/kg feed) on growth performance and nutrient digestibility were tested in two in vivo broiler experiments each arranged in a 2 X 2 factorial design (enzyme x sex in experiment 1 and enzyme x protein level in experiment 2). Each dietary treatment had 12 replicates. In experiment 1, birds were fed 12.7 MJ ME per kg iso-energetic diets in 2 phases. Each diet was fed without or with RPA (C or C+RPA, respectively) to either males or females. In experiment 2, two diets were fed in four treatments. Diet 1 (211 and 200 g CP per kg feed in the starter and grower phases respectively) was fed without or with RPA (NP or NP + RPA, respectively). Diet 2 (200 and 190g CP per kg feed in the starter and grower phases respectively), was fed without or with RPA (LP or LP + RPA, respectively). At the end of each experiment, eight male birds per treatment were randomly selected and used for ileal digestibility measurements. In vitro, RPA significantly increased the degree of protein hydrolysis, solubilisation and digestibility. In males, C+RPA was significantly better than C in WG (2,393g vs. 2,262g) and FCR (1.60 vs. 1.65). In females, C+RPA was better than C in FCR (1.37 vs. 1.39) in the starter phase. The FCR of LP + RPA was significantly better than LP. In experiment 1, RPA significantly increased ileal protein (82.8% vs. 76.9%) and energy (77.8% vs. 70.6%) digestibilities. In LP, RPA significantly improved energy digestibility (76.6% vs. 75.5%). These results suggest that RPA can improve broiler performance by enhancing protein and energy digestibility.