Comparative Effects of Intravenously Single-injected D-[U-~(13)C]Glucose and [1-~(13)C]Sodium Acetate on Time Course Changes in ~(13)C Enrichment of Respiratory CO_2 in Growing Chickens

摘要

This study was carried out to examine which of intravenous glucose or acetate would be rapidly oxidized to CO_2 in growing chickens fed a semi-purified diet. ~(13)C Enrichment of respiratory CO_2 in chickens intravenously single-injected with [1-~(13)C]sodium acetate sharply increased and reached the peak level within 30-40 min, then almost returned to the initial level by 170 min. On the other hand, the peak level in chickens intravenously injected with D-[U-~(13)C]glucose was observed at about 100min and did not return to the initial level even at 4 h. The peak level of ~(13)C enrichment in chickens injected with [1-~(13)C]sodium acetate was about 2.3 times higher than that in chickens injected with D-[U-~(13)C]glucose. The rate of ~(13)C recovered in the respiratory CO_2 for 4h tended to be higher in chickens injected with [1-~(13)C] sodium acetate than in those injected with D-[U-~(13)C]glucose, although the difference was not significant. Rapid appearance of ~(13)C in the respiratory CO_2 from [1-~(13)C]sodium acetate more than D-[U-~(13)C]glucose suggests that cecal acetate is utilized as effective as dietary glucose in the chicken body.