Approaches for Microbiological Characterization of a Landfill Leachate Treatment

摘要

Landfill Leachates are a potential source of soil and water contamination. Biological remediation techniques are most often employed for treating landfill leachates.The nature of microorganisms dwelling in sludge flocs determines process performance.Samples were taken at different steps that constitute the nitrification-denitrification treat ment of leachate in a municipal landfill located in Northern Spain. Process effectiveness was evaluated. Microbiological characterization of samples was performed by means of classical culturing. Also, molecular techniques (i.e., PCR-DGGE and DNA sequencing)were used to characterize the samples. An evolution of the microbial community com position was observed throughout the treatment process. Amplification, electrophoresis, and DNA sequencing steps were completed accurately and some β-proteobacteria related with waste treatment and composting processes could be identified.