Intrahepatic transplantation of cord blood CD34+ cells into newborn NOD/SCID-IL2Rγ null mice allows efficient multi-organ and multi-lineage hematopoietic engraftment without accessory cells

摘要

Advancing the current understanding in experimental and developmental hematopoiesis, hematopoietic transplantation, graft-versus-host-disease (GvHD) and graft-versus-leukemia, immune system, leukemogenic processes as well as testing in pre-clinical models novel cell- and drug-based therapeutic approaches demand the use of xenotransplant systems [1]. Upon discovery of the scid mutation, the immune-deficient non-obese diabetic NOD/SCID mouse was developed, and rapidly became the standard model for hematopoietic xenotransplantation. Several NOD/SCID generations have evolved over the last decade [2,3]. Recently, the NOD/SCID-!L2Rgamma~(null) strain (NSG) has been developed which allows higher engraftment levels and does not develop thymic lymphoma therefore displaying a longer lifespan [4,5].