Denitrification and C, N mineralization as function of temperature andmoisture potential in organic and mineral horizons of an acid spruceforest soil

摘要

A cellular protein, previously described as p55, binds specifically to the plus strand of the mouse hepatitis virus (MHV) leader RNA, We have purified this protein and determined by partial peptide sequencing that it is polypyrimidine tract-binding protein (PTB) (also known as heterogeneous nuclear ribonucleoprotein [hnRNP] I), a nuclear protein which shuttles between the nucleus and cytoplasm, PTB plays a role in the regulation of alternative splicing of pre-mRNAs in normal cells and translation of several viruses. By UV cross-linking and immunoprecipitation studies using cellular extracts and a recombinant PTB, we have established that PTB binds to the MHV plus-strand leader RNA specifically. Deletion analyses of the leader RNA mapped the PTB-binding site to the UCUAA pentanucleotide repeats. Using a defective-interfering RNA reporter system, we have further shown that the PTB-binding site in the leader RNA is critical for MHV RNA synthesis, This and our previous study (H,-P. Li, X, Zhang, R Duncan, L, Comai, and M, M, C. Lai, Proc. Natl, Acad, Sci, USA 94:9544-9549, 1997) combined thus show that two cellular hnRNPs, PTB and hnRNP Al, bind to the transcription-regulatory sequences of MHV RNA and may participate in its transcription.