Epitope analysis of streptococcal M3 antigen-specific monoclonal antibody.

摘要

The binding domains of four monoclonal antibodies (mAbs) (4E10, 2C11, 1E10 and 3H9) specific for M3, the major protein surface antigen of Streptococcus pyogenes, were determined by western blot analysis using truncated M3 polypeptides. The data indicated that epitopes for four mAbs are located within the B repeat block in M3 protein. A sandwich enzyme-linked immunosorbent assay for detecting of M3 protein was established using these mAbs and polyclonal anti-M3 antibody. The technique would be beneficial to our understanding of the epidemiology and to better control of streptococcal diseases.