A field-able RNA extraction and purification procedure.

摘要

This study describes the use of the ChargeSwitch extraction technique as a method amenable to in-field RNA extraction and purification. The modified ChargeSwitch extractions were performed by lysing cells captured from filtration of 30 mL sea water sample spiked with cultured Karenia brevis and sea water samples from regions around a known red tide event. Extractions were compared to traditional laboratory-based silica spin columns by integration with real-time nucleic acid sequence-based amplification. The ChargeSwitch extraction method was equivalent to or was faster than traditional laboratory analysis whilst having the additional advantages of obtaining consistently higher RNA concentrations and requiring no specialized or powered equipment. This method represents a fast and field-able method for RNA extraction from K. brevis and potentially other organisms of public health significance.