Salmonella detection by polymerase chain reaction after pre-enrichment of feed samples.

摘要

A polymerase chain reaction (PCR) procedure was developed for analysis of viable Salmonella spp. in feed samples and scrapings from feed mills. The objective was to develop a cost-effective, user-friendly method that could be performed within ordinary working hours. A single pre-enrichment was enough for most feed raw materials. However, scrapings and pellets required additional pre-enrichment. The results indicate that inhibition of the PCR reaction was correlated with a pH value below 7.0 in the first pre-enrichment. Results of the PCR procedure were in agreement with the established culture-based Nordic Committee on Food Analysis method in spiked samples. The developed PCR procedure can be used as a routine analysis method for feed samples and samples from a Salmonella feed control program based on hazard analysis critical control point principles..