Hydrogen-deuterium exchange and ultraviolet resonance raman spectroscopy of bacteria in a complex food matrix

摘要

The rapid identification and quantification of pathogenic foodborne bacteria is a national research priority. Data collected with a new spectrochemical method suggests that resonance Raman spectroscopy can rapidly and selectively identift and quantify bacteria in foods and other complex biomatrices. This method utilizes hydrogen-deuterium exchange (HDE) to resolve the spectral fingerprints of individual components in the overlapped regions of the ultraviolet resonance Raman spectra of heterogeneous samples. We illustrate this concept with HDE-induced changes in the spectra of bacteria and beef carcass wash samples. The data presented here suggest that the combination of ultraviolet resonance Raman spectroscopy and HDE can potentially establish the identities and quantities of bacteria in heterogeneous samples within seconds to minutes.