SIMULTANEOUS DETERMINATION OF METFORMIN HYDROCHLORIDE IN ITS MULTICOMPONENT DOSAGE FORMS WITH SULFONYL UREAS LIKE GLICLAZIDE AND GLIMEPIRIDE USING HPTLC

摘要

A simple, precise, and accurate high performance thin layer chromalography (HPTLC) method was developed for the simultaneous estimation of melformin with sulfonylureas like gliclazide and glimepiride present in multicomponent dosage forms. This employs a precoaled silica gel 60 F_254 (0.2 mm thickness) on aluminum sheets and a mobile phase ammonium sulfale (0.25%): methanohethyl acetate in the ratio of 10.0:2.5:2.5 (v/v/v), having chamber saturation for 30min at room temperature. The developing chamber was run uplo 8 cm. Validation of the developed HPTLC method was carried out. Good sensitivity for all analyles was observed with UV detection. The method allowed quantitation over the 100-500 ngmL~(-1? range for gliclazide and 1000-5000 ng- mL~(-1? range for metformin hydrochloride in combination I and 300-500ngmL~(-1? range for glimepiride and 150000-250000ng- mL~(-1? range for metformin in combination II. The method has been applied, without any interference from excipients or endogenous substances, for the simultaneous determination of these two combinations in tablets.