SIMULTANEOUS DETERMINATION OF FIVE ACTIVE COMPOUNDS FROM DYSOSMA DIFFORMIS ROOTS BY HPLC

摘要

A simple, rapid, and sensitive high-performance liquid, chromatography (HPLC) method has been developed to simultaneously identify and determine five major active components from Dysosma difformis root, using a Dianionsil octadecyl bonded silica (ODS) C_(18) column (250 mm x 4.6 mm) and Photo-diode array (PDA) detector at 254 nm. Satisfactory separation was achieved by an isocratic elution with 0.1% aqueous phosphoric acid/methanol (40:60, v/v, pH=3.2) at a flow rate of 1.0 mL/min. Four flavonoids (quercetin, kaempferol, rutin, and quercitrin) and a, lignan (podophyllotoxin) in D. difformis root were well separated on the chromatogram within 24 min. Validation of the reverse phase HPLC (RP-HPLC) method showed, good linearity (r~2>0.9989) of the five compounds, which could be analyzed in relatively wide concentration ranges. The relative standard deviation values for intra-day and inter-day precision were less than 5.1%. The contents of these five active components in D. difformis root of different growth locations were also detected.