Separation of Selected Bile Acids by TLC. VIII. Separation on Silica Gel 6OF254 Glass Plates Impregnated with Cu(II), Ni(II), Fe(II), and Mn(II) Cations

摘要

The aim of our study was to examine the following bile acids: cholic acid (C), glycocholic acid (GC), glycolithocholic acid (GLC), deoxycholic acid (DC), chenodeoxycholic acid (CDC), glycodeoxycholic acid (GDC), and lithocholic acid (LC). In the present study, to separate a/m bile acids using adsorption thin layer chrom-atography at 18degC, the glass plates precoated with silica gel 6OF_(254) (#1.05715), were impregnated with 1%, 2,5%, and 5% aqueous solutions of the following salts: CUSO_4, MnSO_4, MSO_4, and FeSO_4. The mixtures of n-hexane-ethyl acetate-acetic acid in the volume compositions: 22:20:5; 25:20:2; 25:20:5, and 25:20:8 were used as mobile phases. These mobile phases were not effective for the separation of bile acids on non impregnated silica gel 6OF_(254) plates at 18degC. The plates impregnated with the salts whose application resulted in (DELTA)R_F > 0.05 and R_s > 1 for all neighboring pairs of examined bile acids were considered the most effective for bile acids separation. It was observed that impregnation of silica gel 6OF_(254) glass plates with aqueous solutions of CuSO_4, MnSO_4, NiSO_4, and FeSO_4 improved the separation of GC/GDC and C/GLC, which separated poorly on glass plates precoated with non impregnated silica gel 6OF_(254) in adsorption TLC at 18degC.