MICROWAVE ASSISTED MICELLAR EXTRACTION-HPLC DETERMINATION OF ANTHRAQUINONE DERIVATIVES IN SEMEN CASSIAE

摘要

In this article, a novel method, microwave assisted micellar extraction coupled with high performance liquid chromatography has been developed for the determination of jive anthraqui-none derivatives (aloe-emodin, rhein, emodin, chrysophanol, physcion) in semen cassiae. Some important parameters, such as thepH value of the solution, microwave assisted micellar extraction time, extraction power, concentration of surfactant Genapol X-080 solution, liquid to solid ratio, and semen cassiae granularity were investigated. Optimum extraction conditions were obtained as follows: 0.1 g 180urn particle size of semen cassiae, 10Yo Genapol X-080 (w/v), pH 3.0, 260 w microwave power, microwave assisted extraction time for 3min, and liquid/solid ratio of 60:1 (mL/g). The method yields linear calibration curves in the concentration range of 1.0 to 40 fig/mL for target analytes, and the detection limits for the five anthraquinone derivatives were between 6.0ng/mL and 39ng/mL. The relative standard deviations (R.S.D.) were less than 1% (n= 6). In addition, the MAME method established in this article is demonstrated to be a simple, economic, and environmentally friendly alternative to conventional organic solvent extraction methods.