Investigation of an Homologous Series of Fatty Acids by TLC. II. Comparison of Separation of Fatty Acids on RP-18 Plates with Different Mobile Phases

摘要

Fatty acids from octanoic acid have been separated on RP-18 plates with and without a concentrating zone using methanol-water, ethanol-water, and n-propanol-water as mobile phases. The cluster analysis for comparison of R_M parameters of investigated acids for chromatographic conditions used was proposed. Comparison of differences between R_F values showed that acids from nonanoic to octadecanoic are separated on the longest distance, using as mobile phase methanol-water in a volume ratio of 95+5 and on RP-18 plates with and without concentrating zone. Moreover, resolution factor values (Rs) calculated suggest that better separations of acids from octanoic to octadecanoic were obtained on RP-18 plates with a concentrating zone and methanol-water (95+5, v/v) as mobile phase.