DIRECT CHROMATOGRAPHIC SEPARATION OF 2-(2-AMINO-1,3-THIAZOL-4-YL)METHYLGLYCINE AND ITS METHYL ESTER ENANTIOMERS USING A CHIRAL CROWN ETHER COLUMN

摘要

Direct liquid chromatographic separation of 2-(2-amino-1,3-thiazol-4-yl) methylglycine and its methyl ester enantiomers was achieved using a chiral crown ether column. The separation was strongly influenced by column temperature and the pH of mobile phase. The method can detect the undesired (+)-enantiomer down to a level of 0.5% and is routinely employed to determine the purity of the desired (-)-enantiomer.