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Genetic Diversity and Biocontrol Potential of Rhizospheric Microbes Isolated from Tomato and Maize

机译:番茄和玉米中根际微生物的遗传多样性和生物防治潜力

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摘要

Sixteen potential microbial agents consisting of seven bacterial isolates and nine Trichoderma isolates were used in study. Among studied 16 microbial culture isolates Bsp 3/aM, 2apa, Mys 21/a, Hasl/c, T2, M5, M29 and M21 showed antagonism against Aspergillus flavus and Fusarium verticillioides and these isolates were positive for root colonization in maize seedlings. Rhizobacterial and Trichoderma sp. diversity was analyzed using sequence and molecular marker based analysis, which failed to group isolates based on antagonism, region of origin and root colonization. Interestingly, in ITS and ISSR diversity analysis two Trichoderma isolates T8 and M3 sliowed low diversity indicating them to be closely related isolates. The study highlighted use of diverse microbial agents showing antagonism and root colonizing potential which can be used in future for field applications and Screening microbial agents with molecular markers will prevent use of closely related isolates and enhance use of diverse and distinct isolates in biocontrol research.
机译:在研究中使用了由七个细菌分离物和九个木霉分离物组成的十六种潜在微生物剂。在研究的16种微生物培养分离株中,Bsp 3 / aM,2apa,Mys 21 / a,Hasl / c,T2,M5,M29和M21表现出对黄曲霉和镰孢镰刀菌的拮抗作用,这些分离株对玉米幼苗的根部定植呈阳性。根瘤菌和木霉使用基于序列和分子标记的分析方法对多样性进行了分析,但未能基于拮抗作用,起源区域和根部定植对分离株进行分组。有趣的是,在ITS和ISSR多样性分析中,两个木霉分离株T8和M3的多样性低,表明它们是密切相关的分离株。这项研究强调了使用具有拮抗作用和根部定殖潜力的多种微生物制剂,这些微生物可以在将来用于田间应用。用分子标记物筛选微生物制剂将防止使用密切相关的分离株,并在生物防治研究中增加使用多种不同的分离株。

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