Antidermatophytic Activity of Some Medicinal Plants, Phytochemical Analysis and Isolation of Active Fractions from Piper longum

摘要

The present study was executed to screen methanolic leaf extracts from 19 plant species for antidermatophytic activity. Based on highest activity, Piper longum was further fractionated to identify the fractions of better activity profile. The extracts and fractions were tested against five dermatophyte species by agar well diffusion and agar dilution methods. Extracts from Aloe vera, Azadirachta indica, Camelia sinensis, Cassia sophera, Clitoria ternatea, Leucas plukentii, Lawsonia inermis, Ocimum basillicum, O. sanctum, O. gratissimum, P betleoides, P ion gum, Solon urn melon gena and Vitex negundo showed antidermatophytic activity (zone of inhibition: 9-42 mm, MIC: 1.25 -10 x 10(4) mu g mL(-1)). Furthermore bioassay of P longum exhibited better activity of sequentially extracted chloroform and methanol extracts (MIC: 5 x 10(3) mu g mL(-1)). Two potential column fractions isolated from the methanol extract of P Ion gum showed MIC of 1.25 x 10(3) - 2.5 x 103 mu g mL(-1) in broth microdilution assay. To the best of our knowledge, there is no scientific report on antidermatophytic activity of P longum so far. The results highlighted future research on these potential plants especially P ion gum to characterize the active constituents.