In vitro Biological Activity of Liquidambar orientalis Mill against Pathogen and Marine Biofilm Microorganisms

摘要

The balsam "liquid storax" of Liquidambar orientalis Mill. that (Sigla) is an endemic tree species for Eastern Mediterranean, was obtained from Gunnucek (Marmaris). It was extracted with n-hexane, CH2Cl2, and MeOH respectively. The n-hexane, CH2Cl2 MeOH extracts were tested at concentrations of 10.0%, 1.0%, 0.4%, 0.2% and 0.1% against two different groups of bacteria: Pathogens and marine biofilm bacteria. Klebsiella pneumonia, Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Pseudomonas aeroginosa, Bacillus cereus, Enterococcus faecalis and Candida albicans were used as pathogen microorganisms whereas,. Pseudoalteromonas marina P. haloplanktis, Alteromonas alvinella, Alteromonas genoviensis Vibrio splendidus, Exiguobacterium homiense, Vibrio lentus were used as marine biofilm bacteria. The antimicrobial test results showed that the MeOH extract of storax has more potent antimicrobial activity than CH2Cl2 and n-Hexane extracts. Inhibitory activities of L. orientalis resin extracts were high against biofilm bacteria, while they were found to be low against pathogenic microorganisms tested.