Column Chromatography and Preparative TLC for Isolation and Purification of Coumarins from Peucedanum verticil/are L. Koch ex DC

摘要

Preparative separation and isolation of coumarins from petroleum ether and methanol extracts of the fruits and roots of Peucedanum verticillare L. Koch ex DC (Umbelliferae = Apiaceae) have been achieved by column Chromatography and normal- and reversed-phase TLC with gradient elution. Isolated coumarins were identified by comparison with standards in analytical TLC and in RP HPLC with gradient elution, by melting point (m.p.) measurement, by UV spectroscopy, by MS, and by ~1H and ~13C NMR spectroscopy. Esculin, umbeliferone, bergapten, xanthotoxin, isoimperatorin, imperatorin, psoralen, and cis-kellactone were isolated for the first time from P. verticillare L. Koch ex DC. Two angular-type pyra-nocoumarins isolated from the roots and fruits of Peucedanum verticillare L. Koch ex DC. were identified as pteryxin and epoxy-pteryxin by MS and ~1H and ~13C NMR.