Application of Densitometry to the Determination of Catechin in Rose-Hip Extracts

摘要

Thin-layer chromatography and densitometry have been used for qualitative and quantitative analysis of catechin in methanolic extracts from 15 taxa of Rosa L. hips. Chromatography was performed on glass silica gel 60 F-254 plates with ethyl acetate-water-formic acid-acetic acid, 25 + 4 + 0.6 + 0.4 (v/v, upper phase), as mobile phase. Chromatograms were scanned in UV light at A = 254 nm. The amount of complex epimers of catechin was determined as (+)-catechin equivalent in the rose extracts investigated. The concentrations of (+)-catechin ranged from 135 to 930 ug g~-1. The highest catechin content was found in R. gallica L., R. penduli-na L., R. tomentosa Sm., R. villosa L. subsp. mollis R. Keller et Gams., and R. dumetorum Thuill. The densitometric method was found to be simple, rapid, and convenient for routine quantitative analysis of catechin in rose hips and may be applied to determination of the compound in other plant materials.