Determination of Gallic Acid After Thin-Layer Chromatographic and Paper Chromatographic Separations

摘要

This paper describes rapid and cost-effective analytical procedures for determination of gallic acid after Chromatographic separation. Optimum conditions were established for separation and assay of gallic acid, salicylic acid, and tannic acid by thin-layer and paper chromatography (PC). Detection was by means of a sensitive spec-trophotometric method based on the coupled redox-complexation reactions occurring in the system Fe(III), gallic acid, and 2,2'-dipyridyl (A_(max) = 522 nm). The procedure was then applied to the determination of gallic acid in a pharmaceutical preparation. Thin layer Chromatographic analysis with densitometric UV detection at A = 280 nm gave comparable results.