Thin-Layer Chromatographic Analysis of Flavonoids, Phenolic Acids, and Amino Acids in Some Croatian Hypericum Taxa

摘要

The flowering tops of Hypericum hirsutum, H. montanum, H. perfo-ratum subsp. angustifolium, H. perforatum subsp. perforatum, and H. perforatum subsp. veronense, collected from different locations in Croatia, were investigated to determine their flavonoid, phenolic acid, and amino acid composition. Thin-layer chromatography (TLC) on silica gel was used for separation and identification of the flavonoids and phenolic acids and TLC on cellulose was used for investigation of the amino acids. By use of these procedures ten flavonoids, three phenolic acids, and sixteen amino acids were detected and identified in the samples investigated. The composition of these compounds depended on the taxa investigated and the locality. Spectrophotometric analysis of the flavonoid content indicated that the three subspecies of H. perforatum are richer in flavonoids than H. hirsutum and H. montanum. H. perforatum subsp. perforatum is the richest in flavonoids, phenolic acids, and amino acids. This is the first time these biologically active compounds have been identified in the some Hypericum taxa.