Quantitative Determination of Olaquindox in Animal Feed

摘要

A quantitative thin layer chromatographic (TLC) method on silica gel, with ethyl acetate-acetone-alcohol, 62 + 20 + 3 (v/v) as mobile phase, has been developed for the determination of olaquiox at ppm levels in animal feed. Preliminary purification consists of extraction with water, adsorption on XAD-4, washing, and elution with acetonitrile. Before the elution step acetylolaquindox is added as internal standard. The residue obtained after evaporation of acetonitrile is disslved in a small volume of metahnol. Quantitation is performed by densitometry at #lambda# = 360 nm.