New Procedure for Analysis of Complex Mixtures by use of Multidimensional Planar Chromatography in Combination with Diode-Array Scanning Densitometry and High-Performance Liquid Chromatography Coupled with Diode-Array Detection

摘要

The purpose of this paper is to report a new procedure for analysis of analytes in complex mixtures by combination of different modes of multidimensional planar chromatography (MDPC) on monolay-er or multiphase plates. The procedure described for separation of complex mixtures of compounds is inexpensive and can be applied to routine analysis of analytes in samples of natural origin, e.g., in water or plant extracts, after preliminary clean-up and concentration, e.g., by solid-phase extraction (SPE). Application of multidimensional planar chromatography (MDPC) and modern fiber optical TLC densitometric scanners with DAD is especially useful for correct identification of components of difficult, complicated mixtures, e.g., pesticides in plant extracts (clofentezine in Herba Thymi).