PUTRESCINE CONTROL OF PEROXIDASE ACTIVITY IN THE INDUCTIVE PHASE OF ROOTING IN POPLAR SHOOTS IN VITRO, AND THE ADVERSARY EFFECT OF SPERMIDINE

摘要

Poplar shoots raised in vitro rooted by 100 % in the presence of an auxin while in its absence they did not root. Putrescine, however, and the inhibitor of spermidine synthase, cyclohexylamine (CHA), which favours putrescine accumulation, were able to promote up to 40 % rooting in the absence of auxin. The inhibitory effect of aminoguanidine (AG), which inhibits diamine oxidase, indicated that the catabolic pathway of putrescine was involved in the rooting process. The inductive phase of rooting in poplar shoots was characterized by an increase of peroxidase activity followed by a rapid decrease. Putrescine and CHA favoured this variation. AG and spermidine had adversary effects.