Somatic embryogenesis and plantlet regeneration from cotyledon explantsisolated from emblings and seedlings of hybrid firs

摘要

Embryogenic tissues have been initiated on cotyledon explants dissected from seedlings or emblings of hybrid firs. Cotyledons of seedling origin (Abies alba x A. cephalonica) gave a relatively low initiation frequency (1.94%). In embling-derived cotyledons (Abies alba x A. cephalonica, Abies alba x A, numidica), the initiation was cell-line dependent and reached values between 1.25 and 24.28%. The established embryogenic cell lines are being maintained in long-term cultures.