Multiplication of Eucalyptus citriodora (L) Hook Through Shoot Bud Induction on the Internodal Portions of Mature Tree Explants

摘要

Nodal explants of Eucalyptus citriodora responded better for high frequency bud break and fast growth in liquid agitated cultures with 4.4#mu#M binzyladenine (BA) and 5.37 #mu#M #alpha#-naphthalene acetic acid (NAA) rather than on semisolid medium.On transfer to MS medium with 1.11#mu#MBA and 5.37#mu#M NAA the sprouted axillary buds showed further elongationgrowth along with a slight xallus and formation of globular structures on the internodal regions.The anatomy of these globular structures revealed that they are shoot buds.These buds elongated into shoots on MS medium with low levels of BA.