Molecular characterisation of Low ODAP Somaclones of Lathyrus sativus

摘要

Somaclones of Lathyrus sativus having low ODAP contents were characterised at molecular level with respect to soluble protein pattern, esterase and ADH isozyme pattern showed the presence of an unique band of Rm 0.73 in Bio L-12 and 15-8-1 and the absence of a band of Rm 0.80 in 15-8-1, as compared to parent and other somaclones. Southern hybridisation of genomic DNA with cNDA clone 29 whowed an extra band of 13.5kb in L-56 and 15-8-1 only . Hybridisation of mitochondrial DNA with mitochondrial specific ati ATPase probe showed differences with the pattern from Bio R-15 being unique. Sone of these dfferences observed will be useful as marker for their identification.