Multiparametric luminescent cell viability assay in toxicology models: A critical evaluation

摘要

Introduction: In cellular viability assays the sole determination of a single parametermight not give precise information on the extent of toxicity. In our study we worked out a multiparametric microplate assay based on bioluminescent ATP quantification, esterase activity-related fluorescence, nucleic acid staining and total intracellular protein measurement from the same sample in MDCK and HepG2 tissue cultures.