The Effect of Oxamate on Fertilization Capacity of Mouse Sperm in vitro

摘要

This study was conducted in vitro to show the effect of oxamate on motility and fertility of the mouse. The spermatozoa were extracted from the caudal part of epididymis. The study animals divided into four groups: (1) control group in TYH medium+5 mg m L~-1 BSA; (2) test groups in TYH medium in which contain 10 Mm oxamate; (3) test groups in TYH medium in which contain 20 Mm oxamate and (4) test groups in TYH medium in which contain 30 Mm oxamate. All four groups were incubated for 90 min to obtain capacitation. Further their motility was checked after incubation time. The mice were super ovulated with PMSG and HCG hormones to obtain oocytes. Total of 600 oocytes were collected and cultured in drops of KSOM medium+5 mg mL~-1 BSA, then for fertilization process received spermatozoa from different groups of mentioned above. After 24-26 h, the rate of fertilization was checked.