Grapevine fleck virus isolates split into two distinct molecular groups.

摘要

Although Grapevine fleck virus (GFkV) has a worldwide distribution, data about its molecular variability are very scarce. Agenome region encoding the central part of the capsid protein gene was amplified from 36 GFkV isolates recovered from a relatively restricted area (Slovakia and the Czech Republic). The nucleotide identities between isolates ranged from 88 to 100%. Phylogenetic analysis showed that the GFkV isolates divided into two groups. Although group I comprised the majority of the isolates, the analysis of the mean nucleotide intragroup divergence showed that group I was less variable than group II. The d_N:d_S ratio was <1 for each group, suggesting anegative selective pressure for amino acid changes in this portion of the genome.