Detection and Identification of a Phytoplasma Related to the 16SrV Group Infecting Chinese Pink in China

摘要

Using universal phytoplasma primer pairs R16mF2/R16mR1 and R16F2n/R16R2, nested polymerase chain reactions were used for phytoplasma detection in Chinese pink with white leaves and stunt symptoms. Sequencing results and restriction fragment length polymorphism (RFLP) analysis of the 1248-bp R16F2n/R16R2 products revealed the phytoplasma belongs to group 16SrV. Phylogenetic analysis showed that the phytoplasma had a close relation to Jujube Witches-broom phytoplasma. This is the first report of a 16SrV-related phytoplasma infecting Chinese pink in China.