An RNA Probe for the Detection of Grapevine virus A

摘要

A specific dot-blot hybridization assay was developed using an in vitro-transcribed non-radioactive RNA probe for the routine detection of Grapevine virus A (GVA) for use as an alternative diagnostic tool for large-scale surveys. GVA was detected in RNA isolated from infected vine plants, plant extracts and tissue prints using this probe however no reaction was observed with healthy plants of genus Vitis.