The effects of Weichangshu tablet on intracellular Ca~(2+) concentration in cultured rat gastrointestinal smooth muscle cells

摘要

The objective of this study was to explore the effects of Weichangshu tablets on free Ca~(2+) concentration of the gastrointestinal smooth muscle cells of rats and the molecular mechanism of the Weichangshu tablets. Cultured SD rat gastrointestinal smooth muscle cells were divided into control group, Cisapride group, Weichangshu group, and control+ ethylene glycol tetraacetic acid (EGTA) group, Cisapride+EGTA group, and Weichangshu+EGTA group. Laser scanning microscope and spectrophotometer detectionwere applied to detect the calcium concentration. The calcium concentrations in Weichangshu group and Cisapride group significantly increased vs. control, and those in Weichangshu group were higher than those in Cisapride group. Ca~(2+) concentration inWeichangshu+EGTA group, Cisapride+EGTA group vs. control+EGTA group were not significantly different. Weichangshu could increase gastrointestinal smooth muscle free Ca~(2+) concentration, and this role may be achieved through the promotion of cells within the flow of calcium ions.