Forster resonance energy transfer (FRET) imaging can show the location of protein-protein interactions in cells, in tissues or throughout an organism. However, it requires exciting a fluorophore with light, which can induce autofluorescenee, damage sensitive tissue and cause photobleaching. Furthermore, because the technique involves the transfer of energy from a donor to an acceptor fluorophore with overlapping spectra, stimulating the donor often inadvertently excites the acceptor.
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