Chiral separation of 3,4-methylenedioxymethamphetamine (MDMA) enantiomers using batch chromatography with peak shaving recycling and its effects on oxidative stress status in rat liver

摘要

This work reports the multimiligram separation of 3,4-methylenedioxy-methamphetamine (MDMA) enantiomers using batch chromatography with peak shaving recycling. The effect of both enantiomers compared to the racemic mixture was examined on the oxidative stress status of rat liver. The enantiomeric purification was performed using a based cyclodextrin chiral selector and methanol:ammonium acetate buffer (pH 6.0, 100 mM) (30:70, v/v) as mobile phase. The average mass rate obtained was 40.0 mg/day, providing 45.0 mg of the (R)-(-)-MDMA (e.r. 99.0%) and 75.0 mg (e.r. 96.0%) of (S)-(+)-MDMA.