This work was dedicated to the development of a new micro immobilized enzyme reactor (IMER) by using an in situ procedure. Arginase was covalently immobilized on an ethylenediamine (EDA) monolithic convective interaction media (CIM) disk (12mm x 3mm i.d.) previously derivatized with glutaraldehyde. The activity of this IMER was investigated by inserting this micro-IMER in a HPLC system. The effect of the arginase inhibitors was evaluated by the simultaneous injection of each inhibitor with the nitro guanidino benzene (NGB) substrate. The relative IC50 values were found in agreement with those derived by the conventional spectrometric method. This arginase micro-IMER system was also used to study the effects of plant-derived products on the arginase activity. The pet ether extract from the stem bark of the plant Ficus glomerata Roxob. and the procyanidin oligomers of cocoa and chocolate inhibit the arginase activity. Our results confirmed the direct effect of some plant extracts on the arginase activity and their interest in therapies for treating several NO-dependent smooth disorders.
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机译：这项工作致力于通过使用原位程序开发新的微型固定化酶反应器（IMER）。将精氨酸酶共价固定在预先用戊二醛衍生化的乙二胺（EDA）整体对流相互作用介质（CIM）盘（12mm x 3mm i.d.）上。通过将该微型IMER插入HPLC系统中，研究了该IMER的活性。通过同时向每种抑制剂注入硝基胍基苯（NGB）底物来评估精氨酸酶抑制剂的作用。发现相对IC50值与通过常规光谱法得出的相对值一致。此精氨酸酶微IMER系统还用于研究植物衍生产品对精氨酸酶活性的影响。从植物无花果榕的茎皮提取的宠物醚提取物。可可和巧克力的原花青素低聚物抑制了精氨酸酶的活性。我们的结果证实了某些植物提取物对精氨酸酶活性的直接影响，以及它们对治疗几种NO依赖性平滑性疾病的治疗方法的兴趣。
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