Time-Resolved Fluorescence HPLC Detection Using Semiconductor Light Sources: Principles and Applications

摘要

Simultaneous static and time-resolved fluorescence detection in HPLC systems using time-correlated single photon counting and modulation techniques is a very recent topic in analytical and physical chemistry. Whereas most commercial systems use simple intensity detection in fluorescence systems or fluorescence spectra with very low sensitivity, here, real-time time-resolved fluorescence counting with on-the-fly data evaluation and display like a typical chromatogram are applied. Both results are independent and characteristic for the fluorescent analytes and exhibit equal sensitivity. For ns resolution, modern semiconductor light sources are used. Modern electronics allow a setup in typical HPLC component size and the measuring procedure requires no adjustment or calibration for the chromatogram. Rapid modification for new applications is possible due to the flexible modular design. The application is patent pending.