Cytogenetic characterization of Donax trunculus (Bivalvia : Donacidae) by means of karyotyping, fluorochrome banding and fluorescent in situ hybridization

摘要

The chromosomes of Donax trunculus were analysed by means of Giemsa staining, chromomycin A3 (CA3), DAPI and fluorescent in situ hybridization (FISH) with an 18S-5.8S-28S rDNA probe. The diploid number is 38 chromosomes and the karyotype consists of nine pairs of metacentric chromosomes, two pairs of submetacentric-metacentric, seven pairs of submetacentric and one pair of telocentric chromosomes. CA3-positive bands are located on eight chromosome pairs and DAPI treatment resulted in uniform staining. Major ribosomal clusters 18S-5.8S-28S are located on the short arm of one submetacentric chromosome pair.