Sequence and molecular analysis of the rpoA cluster genes from Xanthomonas campestris pv. campestris

摘要

The Xanthomonas campestris rpsM(S13)-rpsK(S11)-rpsD(S4)-rpoA(α)-rplQ(L17) cluster, encoding RNA polymerase α-subunit and four ribosomal proteins, reside in a 3164-bp DNA region. The N-terminal sequence of the authentic α-protein determined chemically matches that predicted from the nucleotide sequence. rplQ is monocistronic, instead of being co-transcribed with the other genes as in Escherichia coli. Antiserum against the His-tagged α-protein cross-reacted with the E. coli α-protein.