Fed-Batch Simultaneous Sacchariflcation and Fermentation of Microwave/Acid/Alkali/H_2O_2 Pretreated Rice Straw for Production of Ethanol

摘要

The batch simultaneous saccharification and fermentation (SSF) of microwave/ acid/alkali/H_2O_2 pretreated rice straw to ethanol was optimized using cellulase from Trichoderma reesei and Saccharomyces cerevisiae YC-097 cells prior to the fed-batch SSF studies.The batch SSF optima were 10% w/v substrate,40 deg C,15 mg cellulase/g substrate,initial pH 5.3,and 72 hours.Under the optimum conditions the ethanol concentration and its yield were 29.1 g/L and 61.3% respectively.Based on the optimal batch SSF,the fed-batch SSF was investigated and its operation parameters were optimized.Under its optimal conditions the ethanol concentration reached 57.3 g/L,while its productivity and yield were only slightly less than those in the batch SSF.This suggests that fed-batch SSF is a potential operation mode for effective ethanol production from microwave/acid/alkali/ H_2O_2 pretreated rice straw.