Regionally-selective adhesion and growth of human osteoblast-like MG 63 cells on micropatterned fullerene C-60 layers

摘要

Fullerenes C-60 were deposited on to microscopic glass coverslips using the Leybold Univex-300 vacuum system through metallic masks with rectangle openings. Below the openings, the C-60 formed prominences 128 +/- 8 nm (A), 238 +/- 3 nm (B), 326 +/- 5 nm (C) or 1043 +/- 57 nm (D) in height. Human osteoblast-like MG 63 cells in the cultures on samples A, B and C were distributed almost homogeneously, while on samples D, they were localized almost exclusively in the grooves among the prominences. Thus, fullerene C-60 films can act as substrates for guided cell adhesion and growth.