Cloning of chicken and mouse α_(1b) adrenergic receptor

摘要

A partial cDNA encoding most of the third intracellular loop of the chicken α_(1b) adrenergic receptor subtype, obtained by reverse transcription-polymerase chain reaction (RT-PCR) techniques using degenerate primers derived from mammalian sequences, was used to isolate an α_(1b) adrenergic receptor cDNA from brain. The cDNA encodes a potential protein of 507 amino acids and Northern hybridization of poly(A)~+ RNA from chicken brain of different developmental stages detected a single 3.5 kb transcript. Analysis of receptor expression indicated that the α_(1b) adrenergic receptor is widely distributed in chicken tissues, specially kidney and liver. cDNA and genomic clones encoding sequences of the mouse α_(1b) adrenergic receptor were also isolated.