首页> 外文期刊>Journal of Neuroscience Methods >Effects of ciliary neurotrophic factor and leukemia inhibiting factor on oxytocin and vasopressin magnocellular neuron survival in rat and mouse hypothalamic organotypic cultures.
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Effects of ciliary neurotrophic factor and leukemia inhibiting factor on oxytocin and vasopressin magnocellular neuron survival in rat and mouse hypothalamic organotypic cultures.

机译:睫状神经营养因子和白血病抑制因子对大鼠和小鼠下丘脑器官型培养物中催产素和加压素大细胞神经元存活的影响。

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摘要

Organotypic cultures of mouse and rat magnocellular neurons (MCNs) in the hypothalamo-neurohypophysial system (HNS) have served as important experimental models for the molecular and physiological study of this neuronal phenotype. However, it has been difficult to maintain significant numbers of the MCNs, particularly vasopressin MCNs, in these cultures for long periods. In this paper, we describe the use of the neurotrophic factors, leukemia inhibiting factor (LIF) and ciliary neurotrophic factor (CNTF) to rescue rat vasopressin (Avp)- and oxytocin (Oxt)-MCNs from axotomy-induced, programmed cell death in vitro. Quantitative data are presented for the efficacy of the LIF family of neurotrophic factors on the survival of MCNs in three nuclei, the paraventricular (PVN), supraoptic (SON), and accessory (ACC) nuclei in the mouse and rat hypothalamus.
机译:下丘脑-神经下垂体系统(HNS)中的小鼠和大鼠巨细胞神经元(MCN)的器官型培养已成为对该神经元表型进行分子和生理研究的重要实验模型。但是,很难在这些培养物中长期维持大量的MCN,尤其是加压素MCN。在本文中,我们描述了使用神经营养因子,白血病抑制因子(LIF)和睫状神经营养因子(CNTF)来挽救大鼠血管加压素(Avp)-和催产素(Oxt)-MCNs免受轴索切开诱导的程序性细胞死亡体外。定量数据显示了LIF家族的神经营养因子对小鼠和大鼠下丘脑三个核,室旁(PVN),视上(SON)和副(ACC)核中MCN存活的功效。

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